Enhanced energy savings in enzymatic refining of hardwood and softwood pulp

Abstract Background Pulp refining is an energy consuming, but integral part of paper production with the aim to increase tensile strength and smoothness sheets. Commercial enzyme formulations are used lower requirements by pre-treatment pulp before refining. However, a high number different commercial products available on market containing enzymes varying origin composition, which complicates prediction their behavior, especially using types. Results Endoglucanase-rich were characterized regarding activity at temperatures, resulting in significant decrease above 70 °C. Some preparations additionally contained arabinosidase, xylanase β-glucosidase consequently release xylose glucose from as determined high-performance liquid chromatography. Interestingly, one formulation even showed lytic polysaccharide monooxygenase (LPMO) 3.05 nkat mg −1 . A correlation between endoglucanase specific derivatized cellopentaose (CellG5) substrate performance laboratory PFI (Papirindustriens forskningsinstitut) trials was observed softwood maximum degree values 27.7°SR 32.7°SR. When added purified (31.6°SR), synergistic effects found for cellobiohydrolase II (34.7°SR) or (35.7°SR) Comparison previously obtained results hardwood allowed differences based types be elucidated. Conclusions individual indeed pulp. This difference could predicted after development adapted assay combination CellG5 either sulfate